Persönlicher Status und Werkzeuge

Sprachwahl

Biotechnology

Biotechnology can broadly be defined as ‘the use of biological processes to solve problems or make useful products’. Biotechnology has been practiced by human societies since the beginning of recorded history, in activities such as baking bread, brewing alcoholic drinks and the selective breeding of plants and animals. Modern biotechnology involves precisely modifying the genome of an organism to alter its phenotype for a defined purpose.

Animal biotechnology and biomedicine

Our group is interested in  biomedical applications of animal biotechnology. Recent decades have seen a vast increase in our understanding of the genetic basis of many serious human diseases, because abundant DNA sequence information has been coupled with powerful methods of genetic engineering, principally in mice. Mice however are often of limited value in the preclinical evaluation of new medical techniques. Large animals, such as the pig, can be more useful to research close to the clinic, being similar in size and anatomically and physiologically closer to humans. We are generating genetically-modified pigs that will improve the diagnosis and treatment of serious human diseases, focussing on xenotransplantation and models of human cancers and cardiovascular disease. 

Technology development

Tools for genetic engineering are continually being developed and refined. We are interested in extending new methods to livestock. Technologies we use include multipotent stem cells, transfer vectors for large and multiple transgenes,  site-specific recombination for conditional gene expression, and streamlined gene targeting using RNA-guided endonucleases.

 

Selected publications

Gene targeting directly in rabbit embryos using zinc finger nucleases

Analysis of a spontaneous mutation causing stillbirth in cattle 

Gene targeted modification of the tumour suppressor p53 in pigs 

Pig model of human inherited predisposition to colorectal cancer

Fluorescent reporter pigs for conditional gene expression 

Pigs with conditionally expressed oncogenic KRAS

A porcine model of osteosarcoma

Multi-modified pigs for xenotransplantation

 

Notable older publications

Cloned sheep produced by nuclear transfer

First transgenic animals using nuclear transfer

First gene targeted large animals